Imaging Flow Cytometry
Jacob Garza, PhD
Director, Imaging Flow Cytometry
Senior Research Scientist, Dept. of Neuroscience & Regenerative Medicine
706-721-3280 / email: jgarza@augusta.edu
Amnis ImageStreamX Mk II Imaging Flow Cytometer
The revolutionary ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.
This system comes equipped with the 405, 488, 642, 561, and 785 lasers, autosampler, two cameras, 12 imaging channels and extended depth of field (confocal-like image projection).